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dna microarray hybridization  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna microarray hybridization
    Dna Microarray Hybridization, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+hybridization/DNA/us11753744-139-24-33
    Average 99 stars, based on 1 article reviews
    dna microarray hybridization - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Microarray:

    Article Title: MORC 2 promotes development of an aggressive colorectal cancer phenotype through inhibition of NDRG 1
    Article Snippet: .. To search MORC2 target genes, we undertook DNA microarray hybridization (Affymetrix, Santa Clara, CA, USA) using stable pcDNA3.1 or MORC2‐overexpressed gastric cancer SGC‐7901 cells and found various MORC2 target genes, most of which were downregulated. ..

    Article Title: MORC2 promotes development of an aggressive colorectal cancer phenotype through inhibition of NDRG1.
    Article Snippet: .. To search MORC2 target genes, we undertook DNA microarray hybridization (Affymetrix, Santa Clara, CA, USA) using stable pcDNA3.1 or MORC2overexpressed gastric cancer SGC- 7901 cells5 and found various MORC2 target genes, most of which were downregulated. ..

    Article Title: Changes of global gene expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development.
    Article Snippet: .. DNA microarray hybridization was performed according to the Amino Allyl MessageAmpII aRNA Kit (Ambion Life Technologies, USA, Cat. N: 1753). .. 1 μg purified RNA was used for first and second strand cDNA synthesis. aaRNA was labeled with Cyanine3 (Cy3) and Cyanine5 (Cy5) dyes (Amersham Bioscience, USA).

    Article Title: Profiling invasive Plasmodium falciparum merozoites using an integrated omics approach
    Article Snippet: Amplified ss-cDNAs were generated following the manufacturer’s recommended protocol, quantified using A260/280 spectrophotometer and analyzed on Agilent’s 2100 BioAnalyzer. .. The ss-cDNAs were fragmented and labeled following Nugen’s FL-Ovation cDNA Biotin Module V2 protocol (Nugen, San Carlos, CA) prior to Affymetrix DNA microarray hybridization. .. Hybridization, fluidics and scanning were performed according to standard Affymetrix protocols ( http://www.affymetrix.com ) for the GeneChip® Plasmodium/Anopheles Genome Array.

    Article Title: Transcriptomic data analysis and differential gene expression of antioxidant pathways in king penguin juveniles ( Aptenodytes patagonicus ) before and after acclimatization to marine life
    Article Snippet: .. How data was acquired , Microarray data were obtained by DNA microarray hybridization (Affymetrix GeneChip® Chicken Genome Array). Tissue: pectoralis muscle biopsy of juvenile king penguins excised under general anesthesia.. ..

    Article Title: Microchidia protein 2, MORC2, downregulates the cytoskeleton adapter protein, ArgBP2, via histone methylation in gastric cancer cells.
    Article Snippet: ArgBP2 is an adapter protein that plays an important role in actin-dependent processes such as cell adhesion and migration.. However, its function and regulation mechanisms in gastric cancer have not yet been investigated.. Here, we showed the low expression of ArgBP2 mRNA level in gastric tumor samples and its repressive function in the proliferation, migration, and invasion of gastric cancer cells.

    Article Title: DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
    Article Snippet: .. After DNA isolation, the chromatin interactors or substrates are identified and quantified through decoding of the MN or CA barcode using methodologies such as DNA microarray hybridization or DNA sequencing (Mardis, 2008), e.g. Ion Torrent (Rothberg et al., 2011) or Illumina. (B) DNA Barcoding of the Experimental History of Each MN or CA Library Member. ..

    Article Title: Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis
    Article Snippet: HUVECs were treated with recombinant HMGB-1 (ProSpec-Tany TechnoGene Ltd., Ness Ziona, Israel) at 1 μg/ml and recombinant VEGF (ProSpec) at 50 ng/ml for 20 minutes, 45 minutes, 2 hours, 6 hours, or 12 hours. .. For DNA microarray hybridization, RNA was extracted from HUVECs using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). ..

    Hybridization:

    Article Title: MORC 2 promotes development of an aggressive colorectal cancer phenotype through inhibition of NDRG 1
    Article Snippet: .. To search MORC2 target genes, we undertook DNA microarray hybridization (Affymetrix, Santa Clara, CA, USA) using stable pcDNA3.1 or MORC2‐overexpressed gastric cancer SGC‐7901 cells and found various MORC2 target genes, most of which were downregulated. ..

    Article Title: MORC2 promotes development of an aggressive colorectal cancer phenotype through inhibition of NDRG1.
    Article Snippet: .. To search MORC2 target genes, we undertook DNA microarray hybridization (Affymetrix, Santa Clara, CA, USA) using stable pcDNA3.1 or MORC2overexpressed gastric cancer SGC- 7901 cells5 and found various MORC2 target genes, most of which were downregulated. ..

    Article Title: Changes of global gene expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development.
    Article Snippet: .. DNA microarray hybridization was performed according to the Amino Allyl MessageAmpII aRNA Kit (Ambion Life Technologies, USA, Cat. N: 1753). .. 1 μg purified RNA was used for first and second strand cDNA synthesis. aaRNA was labeled with Cyanine3 (Cy3) and Cyanine5 (Cy5) dyes (Amersham Bioscience, USA).

    Article Title: Profiling invasive Plasmodium falciparum merozoites using an integrated omics approach
    Article Snippet: Amplified ss-cDNAs were generated following the manufacturer’s recommended protocol, quantified using A260/280 spectrophotometer and analyzed on Agilent’s 2100 BioAnalyzer. .. The ss-cDNAs were fragmented and labeled following Nugen’s FL-Ovation cDNA Biotin Module V2 protocol (Nugen, San Carlos, CA) prior to Affymetrix DNA microarray hybridization. .. Hybridization, fluidics and scanning were performed according to standard Affymetrix protocols ( http://www.affymetrix.com ) for the GeneChip® Plasmodium/Anopheles Genome Array.

    Article Title: Transcriptomic data analysis and differential gene expression of antioxidant pathways in king penguin juveniles ( Aptenodytes patagonicus ) before and after acclimatization to marine life
    Article Snippet: .. How data was acquired , Microarray data were obtained by DNA microarray hybridization (Affymetrix GeneChip® Chicken Genome Array). Tissue: pectoralis muscle biopsy of juvenile king penguins excised under general anesthesia.. ..

    Article Title: Microchidia protein 2, MORC2, downregulates the cytoskeleton adapter protein, ArgBP2, via histone methylation in gastric cancer cells.
    Article Snippet: ArgBP2 is an adapter protein that plays an important role in actin-dependent processes such as cell adhesion and migration.. However, its function and regulation mechanisms in gastric cancer have not yet been investigated.. Here, we showed the low expression of ArgBP2 mRNA level in gastric tumor samples and its repressive function in the proliferation, migration, and invasion of gastric cancer cells.

    Article Title: DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
    Article Snippet: .. After DNA isolation, the chromatin interactors or substrates are identified and quantified through decoding of the MN or CA barcode using methodologies such as DNA microarray hybridization or DNA sequencing (Mardis, 2008), e.g. Ion Torrent (Rothberg et al., 2011) or Illumina. (B) DNA Barcoding of the Experimental History of Each MN or CA Library Member. ..

    Article Title: Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis
    Article Snippet: HUVECs were treated with recombinant HMGB-1 (ProSpec-Tany TechnoGene Ltd., Ness Ziona, Israel) at 1 μg/ml and recombinant VEGF (ProSpec) at 50 ng/ml for 20 minutes, 45 minutes, 2 hours, 6 hours, or 12 hours. .. For DNA microarray hybridization, RNA was extracted from HUVECs using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). ..

    Labeling:

    Article Title: Profiling invasive Plasmodium falciparum merozoites using an integrated omics approach
    Article Snippet: Amplified ss-cDNAs were generated following the manufacturer’s recommended protocol, quantified using A260/280 spectrophotometer and analyzed on Agilent’s 2100 BioAnalyzer. .. The ss-cDNAs were fragmented and labeled following Nugen’s FL-Ovation cDNA Biotin Module V2 protocol (Nugen, San Carlos, CA) prior to Affymetrix DNA microarray hybridization. .. Hybridization, fluidics and scanning were performed according to standard Affymetrix protocols ( http://www.affymetrix.com ) for the GeneChip® Plasmodium/Anopheles Genome Array.

    DNA Extraction:

    Article Title: DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
    Article Snippet: .. After DNA isolation, the chromatin interactors or substrates are identified and quantified through decoding of the MN or CA barcode using methodologies such as DNA microarray hybridization or DNA sequencing (Mardis, 2008), e.g. Ion Torrent (Rothberg et al., 2011) or Illumina. (B) DNA Barcoding of the Experimental History of Each MN or CA Library Member. ..

    DNA Sequencing:

    Article Title: DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
    Article Snippet: .. After DNA isolation, the chromatin interactors or substrates are identified and quantified through decoding of the MN or CA barcode using methodologies such as DNA microarray hybridization or DNA sequencing (Mardis, 2008), e.g. Ion Torrent (Rothberg et al., 2011) or Illumina. (B) DNA Barcoding of the Experimental History of Each MN or CA Library Member. ..



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